Uji Aktivitas Antioksidan Esktrak Kasar dan Terpurifikasi Daun Salam (Syzygium polyanthum)
Keywords:
Bay Leaf, Antioxidant, Purified, DPPHAbstract
Bay leaves (Syzygium polyanthum) are one of Indonesia’s biological resources that have long been utilized, particularly as a cooking spice and in traditional therapy. Current use by the public is still largely based on empirical evidence, and scientific validation is still needed to support its application. The aim of this study was to determine the antioxidant capacity of both crude and purified bay leaf extracts. Extraction was carried out using the maceration method with 96% ethanol as the solvent. Purification of the extract was performed using a liquid-liquid extraction method with a solvent ratio of ethanol to n-hexane = 1:1. Phytochemical screening was conducted on both the crude and purified extracts. Antioxidant activity was evaluated using the DPPH method. Free radical scavenging was measured by UV-Vis spectrophotometry at the maximum wavelength. The results of phytochemical screening indicated that both crude and purified extracts contained alkaloids, flavonoids, tannins, and saponins. The maximum absorbance wavelength was found at λ = 514 nm. The antioxidant activity (IC₅₀) of the crude and purified bay leaf extracts was 15.120 ± 0.039 ppm and 13.293 ± 0.039 ppm, respectively. These results indicate that bay leaves possess strong antioxidant potential.
References
Duan, S.-C., Kwon, S.-J., & Eom, S.-H. (2021). Effect of Thermal Processing on Color, Phenolic Compounds, and Antioxidant Activity of Faba Bean (Vicia faba L.) Leaves and Seeds. Antioxidants, 10(8), Article 8. https://doi.org/10.3390/antiox10081207
Esazadeh, K., Ezzati Nazhad Dolatabadi, J., Andishmand, H., Mohammadzadeh-Aghdash, H., Mahmoudpour, M., Naemi Kermanshahi, M., & Roosta, Y. (2024). Cytotoxic and genotoxic effects of tert-butylhydroquinone, butylated hydroxyanisole and propyl gallate as synthetic food antioxidants. Food Science & Nutrition, 12(10), 7004–7016. https://doi.org/10.1002/fsn3.4373
Gulcin, İ., & Alwasel, S. H. (2023). DPPH Radical Scavenging Assay. Processes, 11(8), Article 8. https://doi.org/10.3390/pr11082248
Karlina, N., Kunaedi, A., Ahidin, D., Jannah, U., & Zahiyah, Y. (2023). Antioxidant Activity Test of African Leaves Purification Extract (Vernonia Amygdalina Del) With Dpph Method. Jurnal Farmasi Sains Dan Praktis, 1–10. https://doi.org/10.31603/pharmacy.v9i1.7912
Muflihah, Y. M., Gollavelli, G., & Ling, Y.-C. (2021). Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants, 10(10), Article 10. https://doi.org/10.3390/antiox10101530
Mziouid, A., Chebli, B., Berrabah, M., Chebli, H., Heimeur, N., Bounimi, S., & Mayad, E. H. (2022). Phytochemical screening and antioxidant activity of four Moroccan aromatic plant methanolic extracts and essential oils. Arabian Journal of Medicinal and Aromatic Plants, 8(2), Article 2. https://doi.org/10.48347/IMIST.PRSM/ajmap-v8i2.27129
Nuraeni, S., Supangkat, B., & Iskandar, J. (2022). Kajian Etnobotani Tanaman Rempah sebagai Bumbu, Obat dan Kias. Umbara, 7(1), 27–38. https://doi.org/10.24198/umbara.v7i2.39395
Shukla, R., Jain, N., Rai, G., Pandey, V., Mourya, P., & Pal, B. (2023). Unlocking the potential of bay leaf: Exploring its role as a nutraceutical carrier through ethnomedicinal and pharmacological insights. 3.
Younes, K. M., Romeilah, R. M., El-Beltagi, H. S., Moll, H. E., Rajendrasozhan, S., El-Shemy, H. A., & Shalaby, E. A. (2021). In-vitro evaluation of antioxidant and antiradical potential of successive extracts, semi-purified fractions and biosynthesized silver nanoparticles of Rumex vesicarius. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12293–12293. https://doi.org/10.15835/nbha49112293
Zia, M. P., & Alibas, I. (2021). Influence of the drying methods on color, vitamin C, anthocyanin, phenolic compounds, antioxidant activity, and in vitro bioaccessibility of blueberry fruits. Food Bioscience, 42, 101179. https://doi.org/10.1016/j.fbio.2021.101179
