Pengaruh Gel Topikal SH-MSCs Terhadap Ekspresi Gen TNF-α Pada Tikus Wistar Model Alopesia Yang Diinduksi Fluconazole
DOI:
https://doi.org/10.57218/jkj.Vol4.Iss2.1811Kata Kunci:
Alopecia like, Gel topikal SH-MHSCs, IL-10, TNF-αAbstrak
Alopesia ditandai dengan gangguan siklus rambut, sementara terapi konvensional seperti minoksidil sering menimbulkan efek samping iritasi. Penelitian ini bertujuan menganalisis efek gel topikal sekretom mesenchymal stem cells terkondisi hipoksia (SH-MSCs) terhadap ekspresi gen TNF-α pada model alopesia. Sebanyak 24 ekor tikus Wistar jantan dibagi menjadi empat kelompok: kelompok sehat, kontrol negatif (alopesia + base gel), serta dua kelompok perlakuan yang diberi gel SH-MSCs dosis 100 μL/kgBB dan 200 μL/kgBB. Ekspresi gen TNF-α diukur dengan RT-PCR pada hari ke-22. Hasil menunjukkan penurunan ekspresi TNF-α yang signifikan (p < 0,001) pada kelompok perlakuan, dengan efek tertinggi pada dosis 200 μL/kgBB (0,63 ± 0,20) dibandingkan kontrol (2,54 ± 2,00). Analisis statistik dengan uji One-Way ANOVA dan post-hoc LSD mengonfirmasi perbedaan bermakna antar semua kelompok (p < 0,05). Kesimpulannya, gel topikal SH-MSCs dosis 200 μL/kgBB efektif menekan ekspresi TNF-α dan berpotensi sebagai terapi alternatif alopesia yang aman. Penelitian ini memberikan dasar preklinis untuk pengembangan terapi topikal berbasis sekretom.
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